Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
Figure 2
Subcellular localization of BBE31.
(A) Protease digestion assay. Intact B. burgdorferi N40 cells were incubated with Protease K in the absence (-) or presence (+) of 0.05% Triton X-100. After digestion, cells were lysed and proteins were fractionated by SDS-PAGE. Immunoblots were developed with anti-BBE31 or anti-BB0365 (an inner membrane protein). 0, 20, 200: Protease K concentration is 0, 20 or 200 µg/ml. (B) Indirect immunofluorescence staining. Intact unfixed or methanol-fixed B. burgdorferi N40 were incubated with primary rabbit anti-BBE31 (right panels) or rabbit anti-BB0365 (left panels) antibodies, then incubated with the secondary antibodies Alexa 488-labelled goat anti-rabbit. Magnification, x63. Scale bar represents 20 µm.