Identification of DNA-Damage DNA-Binding Protein 1 as a Conditional Essential Factor for Cytomegalovirus Replication in Interferon-γ-Stimulated Cells
Figure 5
pM27 co-precipitates with DDB1.
(A) STAT2-HA and STAT2−/− cells were infected (5 PFU/cell, 24 h) with indicated MCMV-mutants (wt-MCMV, M27-HA-MCMV or M28-HA-MCMV), lysed and subjected to IP using HA-specific antibody, an isotype or no antibody. Precipitated proteins were analyzed by western blotting. (B) Infection was done as in (A). Cells were lysed and used for HA-specific IP. Precipitated proteins were analyzed by western blotting and by silver staining. (C) STAT2-HA and STAT2−/− cells were infected (5 PFU/cell, 18 h) with M27-Flag-VACV, incubated with MG132 (10 µM, for the last 4 h), lysed and subjected to IP using a Flag-specific antibody or an isotype antibody. Precipitated proteins were detected by western blotting. (D) MRC-5 cells were infected (5 PFU/cell, 18 h) with M27-Flag-VACV, lysed and analyzed by IP with Flag-specific antibodies. The immune complexes were separated by SDS-PAGE and analyzed by western blotting.