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Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio

Figure 2

puf2- parasites transform into early liver stage EEF's in mosquito salivary glands.

A, maximum projection of a z-series scan of infected mosquito salivary glands on day 30 of mosquito infection. B, Development of salivary gland-resident parasites from day 18 to day 30 after mosquito infection; puf2- undergo exo-erythrocytic liver stage metamorphosis with bulging visible at day 22 and complete transformation at day 30. C, Proportion of sporozoites versus EEF-like's of wild type, puf1- and puf2- parasites in mosquito SG's during 13 days of salivary gland infection. Scale bars = 10 µm.

Figure 2

doi: https://doi.org/10.1371/journal.ppat.1002046.g002