Septation of Infectious Hyphae Is Critical for Appressoria Formation and Virulence in the Smut Fungus Ustilago Maydis
Figure 5
Hyphal septation depends on CAR formation.
A: CAR formation in wt filaments is shown by the actomyosin ring marker Cdc15-GFP (green) and co-staining with calcofluor white (red). The magnification shows the formation of the first distal septum (red) while the actomyosin ring started to constrict (green). B: The same settings were used for the AB31Δdrf1 mutant expressing Cdc15-GFP. The region of the bud neck is magnified. C: The constitutive active variant Drf1ΔGBD-GFP (green) was expressed in AB31Δdrf1 and localized in filaments at the site of restored hyphal septation (red). (Scale bars: 15 µm).