Lung Adenocarcinoma Originates from Retrovirus Infection of Proliferating Type 2 Pneumocytes during Pulmonary Post-Natal Development or Tissue Repair
Figure 7
Infectivity of JSRV in proliferating and mitotic arrested cell.
(A) Schematic representation of the experimental design. A JSRV-based vector was derived by transfecting 293T with pJS-EFGFP-MC, pGPP-MX-4CTE and pC-MLV-JSenv as described in the materials and methods. The resulting vector, JS-eGFP, was then used to infect synchronized choroid plexus cells (CP) cultures in the presence or absence of aphidicolin. (B) Histograms showing the DNA content of CP cells with or without Aphidicolin (5 µg/ml for 24 hours). The DNA content was measured by 7AAD staining and flow-cytometry analysis and provides an indication of the cell cycle. The x and y axis represent the relative DNA content and the cell counts. (C) Graph showing the transduction efficiency (expressed as fluorescence forming foci/ml) of JS-eGFP in proliferating or mitotic arrested CP as described in Materials and Methods.