Lung Adenocarcinoma Originates from Retrovirus Infection of Proliferating Type 2 Pneumocytes during Pulmonary Post-Natal Development or Tissue Repair
Figure 2
JSRV infection in lambs and adult sheep.
(A) Schematic diagram of the sheep lungs. Experimental inoculations were performed administering JSRV by bronchoscopy directly into the accessory bronchus. Tissue samples (1 for each lamb and 2 for each adult sheep) were collected from eight areas (delimited by red lines dotted lines in the panel) of the right cranial lobe. (B) Graph showing the mean number of JSRV Env+ clusters per animal as detected by immunohistochemistry in four lambs and four adult sheep (error bars indicate ± SD) 10 days post-infection. (C–E) Immunohistochemistry of JSRV Env+ cells in lung sections of adult sheep (C) and lambs (D–E) 10 days post infection. Env expression (characterized by the intra cytoplasmic dark brown colour) was detected in all experimentally infected lambs but not in adult sheep. (F–I) Phenotype of JSRV infected cells in experimentally infected lambs after 10 day post-infection. Panels F–G show lung sections analyzed by confocal microscopy using antibodies towards SP-C (showed in red) and the JSRV Env (showed in green). Nuclei were stained with DAPI and are shown in blue. Arrows indicate JSRV Env+ cells. Panel H–I show lung sections analyzed by confocal microscopy using antibodies towards CC10 (showed in red) and the JSRV Env (showed in green). Inserts show a larger magnification of the area indicated by the arrows. Scale bars in C = 200 µm; D–E = 100 µm; F–G = 47 µm; H = 30 µm; H = 75 µm.