Compensatory Evolution of pbp Mutations Restores the Fitness Cost Imposed by β-Lactam Resistance in Streptococcus pneumoniae
Figure 7
The percentage of interaction is defined as the ratio between the number of cells co-transformed able to grow in 3-AT selective medium and the total number of cells obtained in medium without 3-AT. The plasmid pair pBT-LGF2/pTRG-Gal11 (BacterioMatch II, Stratagene) was used as a positive control. A) Interaction of PBP2bwt-FtsZ and PBP2b28-FtsZ B) As negative controls, the following plasmid pairs were used: pBT/pTRG carrying codifying sequences of non-interacting proteins, C (-); pBT-empty/pTRG-recombinant, to evaluate the two-hybrid system auto-activation for the target proteins (-)/Autoactivation Target; pBT-PBP2b/pTRG-empty, as non-specific transcription activation control mediated by PBP2bwt or PBP2b28, C (-)/Autoactivation Bait.