The bZIP Transcription Factor MoAP1 Mediates the Oxidative Stress Response and Is Critical for Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
Figure 9
The appressorium-like structure is unable to penetrate the onion epidermis or rice leaf sheath cells.
(A and C) The appressorium-like structure develops at the hyphal tips. The appressorium-like structure was induced by placing the mycelia blocks on the onion epidermis cell for 24 hrs. (Scale bars equal 20 µm). (B and D) Onion epidermis cell penetration assay. The hyphae blocks were inoculated on the onion skin cells for 48 hrs. Penetration was observed using DIC microscopy. (Scale bars equal 20 µm). (E and F) Statistical analysis of appressorium-like structure development. A total of 300 hyphal tips were examined and counted. Dark column indicates Guy11, white column indicates the Mossadh or Moact mutant, and gray column indicates the Mossadh/SSADH or Moact/ACT complement strain. Error bar represents standard deviations and asterisks indicate that the differences were significant. (G) Rice leaf sheath penetration assay. Appressorium like structure formed by the Mossadh mutant and Moact mutant are incapable of penetration into the rice leaf sheath cells 48 hrs post injection (Scale bars = 20 µm).