The bZIP Transcription Factor MoAP1 Mediates the Oxidative Stress Response and Is Critical for Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
Figure 2
MoAP1 disruption leads to abnormal conidial morphology and reduced conidiation.
(A) Development of conidia on conidiophores is affected by Moap1 deletion. Strains grown on RDC medium for 7 days were examined by light microscopy. Bars equal 100 µm. (B) Statistical analysis of conidia production. The conidia produced by the wild type strain (Guy11), the mutants and complemented strains grown on RDC medium for 10 days were collected, counted, and analyzed by Duncan analysis (p<0.01). Asterisks indicate significant differences among Guy11, the Moap1 mutants and complemented strains. Error bar represents standard deviation. (C) Conidium morphology. Conidia were harvested from RDC medium, diluted to 1.0×105 spore/ml, and observed by light microscopy. Bars equal 10 µm. (D) Conidia size comparison. The conidia sizes were determined as width by length from 150 conidia of each strain. Asterisks indicate that the difference is statistically significant. Error bars represent standard deviations.