Evidence That Intracellular Stages of Leishmania major Utilize Amino Sugars as a Major Carbon Source
Figure 5
Accumulation of hexosamine phosphates in GlcNAc-fed Δgnd parasites.
(A) Concentration of intracellular sugar-phosphates in WT and Δgnd parasites after cultivation (4hr) in the presence of Glc or GlcNAc. (B) L. major wild type and Δgnd promastigotes were suspended in hexose-free CDM supplemented with or without 13 mM GlcNAc. After 4 hr, the medium was supplemented with 13 mM [U-13C]-Glc and parasites harvested at the indicated time points. The rate of synthesis of Glc6P (indicated by percent Glc6P labeled with 13C), an indicator of glycolytic flux, was determined by GC-MS.