Toxoplasma gondii Lysine Acetyltransferase GCN5-A Functions in the Cellular Response to Alkaline Stress and Expression of Cyst Genes
Figure 5
TgGCN5-A is enriched at genes up-regulated during alkaline pH stress.
Toxoplasma parasites stably expressing FLAG-tagged TgGCN5-A were maintained in HFFs with normal (pH 7.0) or alkaline (pH 8.2) medium prior to harvesting for qChIP using anti-FLAG antibody. Immunoprecipitated DNA was examined using primers to pH responsive genes phosphatidylinositol 3- and 4-kinase (PI3K), and protein kinase (PK) (A), or housekeeping gene controls, GAPDH and actin, ACT (B). Results are represented as a ratio of ChIP/Input DNA. The difference between pH 7 and pH 8 for PI3K is statistically significant (p = 0.02, Student's t-test).