HapX Positively and Negatively Regulates the Transcriptional Response to Iron Deprivation in Cryptococcus neoformans
Figure 5
The hap3Δ and hap5Δ mutants have a smaller capsule than WT or hapXΔ cells.
A. Cells were cultured in low-iron medium (LIM) at 30°C for 48 h. Capsule formation was assessed by India ink staining for the WT strain, the hap3Δ, hap5Δ, hapXΔ, cfo1Δ, hap3Δ cfo1Δ, hap5Δ cfo1Δ, and hapXΔ cfo1Δ mutants, as well as the reconstituted strains. Bar = 10 µ. B. Fifty cells from each strain were measured to determine cell diameter and capsule radius. The capsule sizes of hap3Δ, hap5Δ, hap3Δ cfo1Δ (both #1 and #2) and hap5Δ cfo1Δ (both #54 and #61) cells were statistically different (t-test, p<0.001) from the sizes for cells of the WT strain, the hapXΔ cfo1Δ mutants and the reconstituted strains. Each bar represents the average of 50 measurements with standard deviations.