CpG-Methylation Regulates a Class of Epstein-Barr Virus Promoters
Figure 8
Zta transactivates CpG-methylated promoters with superior efficiency.
Promoters of eleven different genes (Table S3 in Text S1) were inserted into a synthetic luciferase reporter plasmid free of CpG-dinucleotides [39]. Reporter plasmids were either used after isolation from E.coli and thus free of methylated CpG dinucleotides (unmethylated, white) or after complete in vitro CpG-methylation (black) and co-transfected with a BZLF1 expression plasmid or with a negative control DNA as indicated (none). After data normalization to a luciferase control plasmid free of promoter elements, the x-fold differences in the different data sets were calculated as shown.