Role of Acetyl-Phosphate in Activation of the Rrp2-RpoN-RpoS Pathway in Borrelia burgdorferi
Figure 5
Influence of overexpression of wild-type or mutated version of the Rrp2 N-terminal receiver domain on Rrp2 activation.
(A) Schematic diagram of predicted Rrp2 domain structure and various versions of overexpressed N-terminal receiver domains. D52 is the putative phosphorylation site. (B) Immunoblot of wild-type strain (lane 1), the strain carrying the shuttle vector only (lane 2), the strain with overexpression of Rrp2-N (lane 3), the strain with overexpression of Rrp2-N(D52A) (lane 4), and the strain with overexpression of Rrp2-N(D52E) (lane 5). Cultures were grown to late logarithmic phase at 35°C. Pooled antibodies/antisera against Rrp2, FlaB, and OspC were used. Bands corresponding to each protein were labeled on the right. (C) qRT-PCR analysis of ospC expression in various strains shown in (B). Levels of ospC transcript were normalized per 1000 copies of flaB in each sample.