A Systems Immunology Approach to Plasmacytoid Dendritic Cell Function in Cytopathic Virus Infections
Figure 2
MHV infection and IFN response kinetics in vitro.
Experimental data (open symbols) represent the geometric mean ±SEM. Virus titers (red), IFN-α concentration (green) and the fraction of live cells out of the total initial target cell number (black) were used to calibrate the model. (A) In vitro MHV infection of pDCs (MOI = 1). Fraction of infected cells in the population of live cells is shown in blue. (B) MHV infection in pDCs derived from ifnar−/− mice. Fraction of infected cells in the population of live cells is shown in blue. Experimental validation had been performed using infection of pDCs with EGFP-recombinant MHV (blue circles). (C) MHV replication in wild-type Mφs. The data on the amount of secreted IFN-α were used to validate the calibrated model (green circles). (D) Effect of IFN pre-treatment (500 IU of IFN-α, green line) on MHV replication in wt Mφs.