Endothelial Galectin-1 Binds to Specific Glycans on Nipah Virus Fusion Protein and Inhibits Maturation, Mobility, and Function to Block Syncytia Formation
Figure 3
Galectin-1 interferes with lateral movement of NiV-F on the plasma membrane.
A, NiV-FGFP expression on the surface of two individual Vero cells. B, NiV-FGFP promotes cell fusion when transfected into Vero cells with NiV-G. (20×) C, Galectin-1 inhibits fusion mediated by NiV-FGFP. Fusion of Vero cells in the absence (−) or presence (+) of 20µM galectin-1 was measured as in Fig. 1. D, Galectin-1 inhibited fluorescence recovery after photobleaching. NiV-FGFP transfected Vero cells were treated with buffer control (black line with black squares) or 20µM galectin-1 (grey line with black triangles), and a portion of the membrane was bleached and measured for fluorescent recovery (y-axis) as a function of time in seconds (x-axis). Data are mean ± SD of six replicate measurements from one of two independent experiments.