Integration Preferences of Wildtype AAV-2 for Consensus Rep-Binding Sites at Numerous Loci in the Human Genome
Figure 5
Statistical analysis of distances of integration sites from human Rep-binding motifs.
AAV integration sites of different data sets were analyzed for the proximity to the next Rep binding sites. Calculations were performed with the following putative Rep binding sites: GAGC GAGC (A); GAGT GAGC (B); GAGY GAGC GAGC (C); GAGC GAGC GAGC, one mismatch (D), or two mismatches allowed (E), and GAGY GAGC GAGA (F). Average distances of integration sites from putative Rep binding sites are displayed as mean +/− S.D. The levels of significance are marked by asterisks. P-values >0.05 were not considered statistically significant. P-values <0.01 were considered highly significant. For the analysis of wildtype AAV integration data a PvuII-related control set was used in addition to the random control set.