Integration Preferences of Wildtype AAV-2 for Consensus Rep-Binding Sites at Numerous Loci in the Human Genome
Figure 2
Chromosomal distribution of AAV-2 integration sites.
DNA of AAV-2 wildtype infected Hela cells was analyzed for viral integration by the LSM-PCR-method. DNA sequence data from 117 cloned AAV-chromosome junctions were assigned to unique loci. (A) Distributions of junctions on individual chromosomes are shown as percentage of the total 117. (B–D) AAV integration sites drawn to scale for chromosome 19 (B), 5 (C), and 3 (D). Shown are the chromosome ideograms and enlarged bands of hotspots found on chr. 19q13.42 (AAVS1) and chr. 5p13.3 (AAVS2). Solid arrows represent sites detected upon wildtype AAV-2 infection. Open arrows represent junctions stemming from cotransfection of AAV vector- and Rep-expression plasmids.