The SOCS-Box of HIV-1 Vif Interacts with ElonginBC by Induced-Folding to Recruit Its Cul5-Containing Ubiquitin Ligase Complex
Figure 5
15N-HSQC spectrum of protonated EloBC (A) or uniformly-deuterated EloBC bound to unlabelled wild type Vif SOCS-box protein (B). The best spectra available were used for illustration, but similar spectra were recorded on several occasions using different protein preparations. The spectrum of free EloBC shows some well dispersed peaks, but also significant overlap exists between 7.5 and 8.5 ppm in the 1H dimension, indicative of an unstructured domain. The spectrum of bound EloBC is much better resolved, with more peaks corresponding to well-folded domains.