Distinct External Signals Trigger Sequential Release of Apical Organelles during Erythrocyte Invasion by Malaria Parasites
Figure 5
Expression of EBA175 on merozoite surface detected by immunofluorescence assay (IFA) in response to changes in ionic conditions.
Expression of microneme protein EBA175 on surface of P. falciparum merozoites isolated in IC buffer (IC) (A) or following transfer to EC buffer (EC) (B) or following transfer to EC buffer after pre-treatment with BAPTA-AM (EC + BA) (C) was detected by IFA using anti-EBA175 rabbit sera followed by FITC-conjugated anti-rabbit IgG goat sera (green). Nuclear DNA was counterstained with DAPI (blue). Bright field, bright field merged with DAPI staining and merged fluorescence images (DAPI and FITC) are shown. Black bar indicates 2 µm.