The T3SS Effector EspT Defines a New Category of Invasive Enteropathogenic E. coli (EPEC) Which Form Intracellular Actin Pedestals
Figure 2
Wave2 and Abi1 are localized to membrane ruffles and lamellipodia induced by ectopically expressed EspT.
Actin was stained with Oregon Green phalloidin (Green), Wave2 was detected with a polyclonal rabbit antibody (Red) and Abi1 was visualized using a mouse monoclonal antibody (Yellow). Mock transfected cells did not display any significant actin structures and Wave2 and Abi1 were localized diffusely in the cytoplasm. HeLa cells transfected with pRK5::espT exhibit prominent membrane ruffles on their apical surface which were enriched with Wave2 and Abi1. Ectopic expression of EspT in Swiss 3T3 cells resulted in formation of distinctive lamellipodia to which Wave2 and Abi1 were extensively recruited and co-localized.