Mycobacteria Exploit Host Hyaluronan for Efficient Extracellular Replication
Figure 2
Effect of hyaluronan on BCG growth in carbon-starved 7H9 media.
(A) (B), BCG-Luc was cultured in carbon-starved 7H9 media (7H9), or carbon-starved 7H9 media supplemented with 500 µg/ml of HA (7H9+HA), heparin (7H9+Hep), chondroitin sulfate C (7H9+Cho), heparan sulfate (7H9+HS), or glucose (7H9+Glu) at 37°C. Growth of BCG was monitored by luciferase activity. The results are expressed as mean±the standard deviation (n = 3). For statistical analysis, a two-way ANOVA with Bonferroni Post tests were used to obtain P-values for each time point, comparing the various growth conditions to the control. *P<0.01. (C), Uptake of 3H-hyaluronan (HA) by BCG in carbon-starved 7H9 media. Live and heat-killed BCG cells were cultured in carbon-starved 7H9 media in the presence or absence of 3H-labeled hyaluronan for 4 or 7 days. The uptake of 3H-laveled hyaluronan was measured by a gamma counter.