Rab6 and Rab11 Regulate Chlamydia trachomatis Development and Golgin-84-Dependent Golgi Fragmentation
Figure 5
Loss of p115 induces Golgi fragmentation in infected cells independently of Rab6A and Rab11A.
A) Control, Rab6A KD and Rab11A KD cells were transiently silenced for the expression of p115 by siRNA transfection. Control cells were additionally transfected with an siRNA targeting Luciferase. At 3 d p.t. cells were infected with C. trachomatis (MOI 3). At 44 h p.i., cells were lysed and infectious bacteria were titrated on HeLa cells. IFU/ml expressed in log scale B) Control, Rab6A KD and Rab11A KD depleted cells were infected with C. trachomatis at an MOI 1. Infected cells were fixed and stained for GM130 (red channel) and chlamydial LPS (blue channel). shRNA against Luciferase was used as a control. Stable KD cells express GFP as a marker of shRNA expression. Samples were analysed at a LSCM. Merged pictures are shown. Images are representative of n = 3. Scale bar, 10 µm. C) Quantification of Golgi fragmentation. Absolute numbers of Golgi elements/cell are depicted. (A, C) Values were obtained from three independent experiments and are mean±SE.