Lethal Influenza Virus Infection in Macaques Is Associated with Early Dysregulation of Inflammatory Related Genes
Figure 5
The 1918 and VN/1203 viruses differentially regulate the expression of inflammation and cell death related genes.
(A) Venn diagram analysis was performed on the set of 428 genes that were differentially regulated by both viruses at 24 h p.i. All infected samples were compared to genotype-matched mock-infected samples via microarray analysis. Replicate samples were then pooled and error-weighted in silico. These genes were up-loaded into Ingenuity Pathway Analysis and categorized into functional categories that are included to the right of the heat map. (B) Biological network analysis of the top two functional categories: immune response (p-value = 2.42E-22) and inflammatory response (p-value = 1.16E-16) determined by Ingenuity Pathway Analysis. This analysis highlights a subset of genes that were up-regulated by both viruses (green shading) and a subset of genes that were anti-coregulated, that is up-regulated during 1918 infection but down-regulated during VN/1203 infection (blue shading). Boxed genes in the diagram highlight the anti-coregulation of the inflammasome components NLRP3 and IL-1β respectively. Included heat map illustrates the expression of IL1β and NLRP3. Yellow color indicates up regulation, blue indicates down regulation.