Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages
Figure 5
Transmission electron microscopic and IA-SEM imaging of Jurkat T cells infected with the VSV-G pseudotyped 29/31 HIV-1 Gag matrix mutant.
(A, B) Selected projection TEM images from a 100 nm thick section obtained from fixed, osmium-stained, plastic-embedded cells. Small vacuolar compartments, some containing viruses can be visualized in the interior of the cell. (C) A single IA-SEM image from the interior of the same block used to obtain the TEM images in panel (A). A complete stack of images showing the distribution and closed shapes of the compartments through this and another cell is included in Videos S8 and S9. (D) Rendering of the membrane conpartments in the interior of the cell illustrating that they are closed, and not connected to the cell surface irrespective of whether or not they appear to contain viruses (red). Scale bars in all panels are 1 µm wide.