Evolutionarily Conserved Herpesviral Protein Interaction Networks
Figure 5
Core interactions are conserved.
(A) Histogram indicating the percentage of interactions confirmed either by Y2H, CoIP or Y2H and CoIP. We tested in total 92 interactions in HSV-1, mCMV and EBV predicted from 55 interactions detected in KSHV between proteins conserved in at least one other species. For all species more than 50% of the predicted interactions could be confirmed by CoIP, while the numbers confirmed by Y2H were significantly lower. (B) Illustrates how the discovery of new interactions decreases for each new interactome. Average number of new interactions found after 1, 2, 3, 4 and 5 Y2H screens (red points) is plotted, where averages are taken over all possible sequences of Y2H screens. The green line shows a linear best fit line. While there is a clear decrease in the number of new interactions discovered for each additional Y2H analysis, there seems to be a significant number of interactions still to be found.