Modulation of Neutrophil Function by a Secreted Mucinase of Escherichia coli O157∶H7
Figure 5
StcE inhibits neutrophil chemotaxis.
(A) Neutrophils labeled as in figure 3C were treated with 25 µg/mL StcE or E435D in the upper chamber of Fbg-coated transwell inserts, and media with or without chemoattractant (100 nM fMLP) placed in the lower chamber. Cells were allowed to migrate for 210 min and neutrophils in the lower chamber were quantified and normalized as described. Data shown are mean±S.E.M. of three independent experiments. **, p<0.01 compared to vehicle with chemoattractant by one-way ANOVA using Dunnett post test. (B) Experiments were performed as described in (A), with 50 µg/mL protein and monolayers of HMVEC-L grown on collagen-coated transwell inserts. Data are mean±S.E.M. from six independent experiments analyzed as in (A).