Epstein-Barr Nuclear Antigen 1 Contributes to Nasopharyngeal Carcinoma through Disruption of PML Nuclear Bodies
Figure 5
Effect of USP7 silencing on PML degradation by EBNA1.
(A) CNE2E cells expressing EBNA1 were transfected with siRNA against USP7 or GFP (negative control) then stained for USP7 and PML. Exposure times of image capture were constant for all samples with the same antibody treatment. (B) Equal amounts of cell lysates from (A) were analysed by Western blotting with the indicated antibodies. siUSP7-1 and siUSP7-2 are duplicate samples treated with siRNA against USP7. (C) CNE2 cells were transfected with siRNA against GFP or USP7 (left panel) then were transfected with EBNA1 expression plasmid pc3OripE and stained for EBNA1 and PML (right panel). Exposure times of image capture were constant for all samples with the same antibody treatment. (D) Equal amounts of cell lysates from (C) were analysed by Western blotting after pretreatment with siGFP or siUSP7 followed by EBNA1 expression.