Epstein-Barr Nuclear Antigen 1 Contributes to Nasopharyngeal Carcinoma through Disruption of PML Nuclear Bodies
Figure 2
Transient expression of EBNA1 and EBNA1 mutants in CNE2 cells.
(A) CNE2 cells were transiently transfected with a plasmid expressing EBNA1 or EBNA1 mutants Δ325–376 or Δ395–450, then stained for EBNA1 and PML. Both EBNA1-expressing and nonexpressing cells are shown 48 hrs post transfection. Exposure times of image capture were constant for all samples with the same antibody treatment. (B) Numbers of PML NBs per cell were counted 48 hours after expression of wildtype EBNA1. Cells were categorized into low and high EBNA1 expression depending on the intensity of EBNA1 staining. ** indicates 0.0001<p<0.001 and *** indicates p<0.0001 relative to untransfected cells. (C) The number of PML NBs were counted for all cells in (A) expressing wildtype or mutant EBNA1 proteins.