CD4-Specific Designed Ankyrin Repeat Proteins Are Novel Potent HIV Entry Inhibitors with Unique Characteristics
Figure 7
Human CD4 specific DARPins can crossreact with macaque CD4 and inhibit entry of SIV.
(A) PBMC from rhesus macaques were incubated with the indicated DARPins at 4°C, labeled with an anti-His-tag antibody and detected by flow cytometry. MFI (mean fluorescence intensity) of DARPin staining on CD3+ cells, corrected for the negative control is shown. Standard deviations are indicated and represent n = 4 animals. (B) DARPin 25.2 potently inhibits entry of SIVmac239 in macaque PBMC. Shown are the IC90 values which were derived from neutralization assays performed using DARPin 25.2 and the control E3_5 on PBMC from three different animals.