Cellular Proteins in Influenza Virus Particles
Figure 2
Confirmation of host protein incorporation into influenza virions derived from different cell lines.
Influenza A/WSN/33 virus was purified from the supernatant of infected A549 and Vero cells. 2 ug of purified virus derived from A549 and Vero cells (lane 1 and 2, respectively) and 10 ug of cellular extracts from uninfected A549 and Vero cells (lanes 3 and 4, respectively) were subjected to western blot analysis with antibodies against the following proteins: (A) Influenza hemagglutinin (HA0 and HA1 are visible), (B) Beta actin, (C) Annexin A5, (D) Cyclophilin A. Numbers to the left are molecular weight markers.