agr-Mediated Dispersal of Staphylococcus aureus Biofilms
Figure 2
Detachment of S. aureus biofilms with AIP.
Biofilms (strain AH500) were grown in flow cells for 2 days. Either (A) 1 mL of buffer (100 mM phosphate [pH 7], 50 mM NaCl, 1 mM TCEP) or (B) 1 mL of 20 µM AIP-I in buffer was diluted 1000-fold into the biofilm growth media. The biofilm integrity was monitored with CLSM for 2 more days. Each side of a grid square in the image reconstructions represents 20 µM. (C) Effect of AIP-I addition on number of detached bacteria in the effluent medium from flow cell biofilms. The plot depicts CFU/ml in effluents from biofilms, and the black squares (▪) represent AIP-I addition and the black circles (•) represent buffer addition to the biofilm. Graph shows the mean of 3 effluent collections from 1 experiment, error bars show SEM.