Identification of a Bacterial-Like HslVU Protease in the Mitochondria of Trypanosoma brucei and Its Role in Mitochondrial DNA Replication
Figure 5
Effect of TbHslU1+2 double RNAi on free minicircle replication intermediates.
(A). Total DNA was fractionated on an agarose/ethidium gel and a Southern blot was probed for minicircles. N/G, nicked/gapped minicircles; L, linearized minicircles; CC, covalently-closed minicircles, *, nonspecific hybridization to nuclear DNA. The nicked/gapped minicircles form a doublet with the lower component possibly linearized minicircles. Since it is present prior to RNAi induction (day 0), it is likely unrelated to RNAi. (B). Quantitation (by Phosphorimager) of bands from A. The nicked/gapped species includes both components of the doublet.