Cationic Amino Acid Transporter-2 Regulates Immunity by Modulating Arginase Activity
Figure 5
TH1/TH2 cytokine responses are reduced in infected CAT2−/− mice.
A. Leukocytes derived from the granulomatous livers of 8 wk S. mansoni infected mice were stimulated with PMA/Ionomycin/BFA for 3 hr and then stained for CD4, IFN-γ, IL-5 and IL-13. The data show the percentage of CD4+ lymphocytes producing a specific cytokine. Results for individual mice are shown and statistically significant differences are indicated in the figure. B. Frequency of non-CD4+ lymphocytes producing specific cytokines. C. Relative mRNA levels of ifn-γ, il-5, il-13, il-4, il-10 in S. mansoni infected liver tissue were determined by real-time Q-RT-PCR (wk 8,12 and 24), by normalizing mRNA levels to expressed, endogenous HPRT mRNA. Fold increases in relative mRNA levels were determined by the ratio of normalized infected mRNA to uninfected mRNAs. Results from individual mice are shown. The * symbol denotes significant differences between WT and KO mice at that time point, p<0.05.