SNARE Protein Mimicry by an Intracellular Bacterium
Figure 6
CT813 can interact with host SNAREs.
GFP-SNARE proteins (V3 = Vamp3, V4 = Vamp4, V7 = Vamp7, V8 = Vamp8, Longin = Vamp7 without its SNARE motif) were immunoprecipitated from HeLa cells co-expressing CT813-His* or empty vector (pQE). The top left panel (anti-His blot) shows the level of expression of CT813-His* in cell lysates, and the bottom right panel (anti-GFP blot) shows immunoprecipitation of GFP-tagged proteins. CT813-His* co-immunoprecipitated with Vamp7 and Vamp8 (top right panel, anti-His blot), and not with Vamp3, Vamp4, GFP or GFP-Longin. Absence of immunoprecipitation with Vamp3 might be due to insufficient expression level of both CT813-His* and GFP-Vamp3, which were consistently low in these experiments.