SNARE Protein Mimicry by an Intracellular Bacterium
Figure 5
IncA plays a predominant role in SNAREs recruitment to the inclusion.
A, The recruitment of transfected GFP-Vamp8 (left) around the inclusion membrane (labelled with antibodies against the inclusion protein Cap1, middle) is reduced in cells infected with a IncA defective mutant strain (Ds5058, bottom panels) compared to wild type (top panels). B, The percentage of cells (n>100) in which the indicated GFP-SNARE proteins encircle the inclusions is reduced in cells infected with the IncA defective mutant strain (grey) compared to wild type (black). Coverslips were analyzed in duplicate by different investigators, and the means and standard deviations of the two resulting counts are shown. C, The percentage of cells (n>100) in which the indicated endogenous SNAREs encircle the inclusions in control cells (black), cells overexpresing C. caviae IncA (dark grey) or Δ75CtrIncA (light grey) is shown. The means and standard deviations of two experiments are shown.