Hemolytic C-Type Lectin CEL-III from Sea Cucumber Expressed in Transgenic Mosquitoes Impairs Malaria Parasite Development
Figure 2
Binding of CEL-III to Ookinetes Inhibits Parasite Development In Vitro
(A) Binding of CEL-III to ookinetes. CEL-III was added to cultured ookinetes purified from P. berghei–infected mouse blood. Bound CEL-III was detected with FITC-labeled anti-mouse IgG following mouse anti-CEL-III antibody by fluorescence microscopy (panels 1 and 2) (CEL-III (+)). As a negative control, ookinetes were incubated with FITC-labeled anti-mouse IgG following mouse anti-CEL-III antibody without CEL-III (panels 3 and 4) (CEL-III (−)). Panels 1 and 3, phase contrast; panels 2 and 4, FITC. Arrows indicate cultured ookinetes. Scale bars are 10 μm.
(B) Effect of CEL-III on ookinete development in vitro. P. berghei–infected mouse blood was cultured for ookinetes for 24 h at 19 °C. CEL-III was added at initiation of the culture at various concentrations. Data are expressed as number of ookinetes relative to medium alone (100%). Results are the mean of two independent experiments, and bars represent standard errors of the mean.