Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Figure 2
Southern Blot of P. falciparum LipB Mutants
Genomic DNA was isolated from wild-type (P. falciparum 3D7 and D10) as well as four independent LipB mutant clones (KO1–1, KO1–2; KO2–1 and KO2–2). The DNA was digested with NdeI and separated on a 0.8% agarose gel before blotting to nylon and probing with the LipB coding region. The band shown in lanes 1 and 2 corresponds to the endogenous LipB gene locus (2.8 kb). The 2.8 kb band disappears and is replaced by two bands diagnostic for the disruption of the endogenous LipB locus (3.7 kb and 4.9 kb) in all four cloned parasite lines (lanes 3–6).