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Release of Hepatic Plasmodium yoelii Merozoites into the Pulmonary Microvasculature

Figure 2

Merozoite Release by Merosome Formation

(A) Intravital microscopy of merosomes budding from an EEF. Note that a few individual merozoites are located in the vicinity of the EEF (see Videos S1S3). Bar = 10 μm.

(B) Semithin Epon section showing an EEF that has lost contact with the surrounding parenchyma (arrows). Bar = 10 μm.

(C) Projection of an EEF in the process of disintegration; note that the cell releases individual merozoites into the environment (see Video S4). Bar = 10 μm.

(D) Electron micrograph showing merozoites located free in a liver sinusoid. The apical pole of one of the merozoites is in close contact with an erythrocyte (insert). E, erythrocyte; *, merozoites. Bar = 1 μm.

(E) Liver sinusoid containing free hepatocyte mitochondria (arrows) and a free merozoite. E, erythrocyte; *, merozoite. Bar = 5 μm.

Figure 2

doi: https://doi.org/10.1371/journal.ppat.0030171.g002