The Transcription Factor Mrr1p Controls Expression of the MDR1 Efflux Pump and Mediates Multidrug Resistance in Candida albicans
Figure 6
Identification of Mrr1p Target Genes by DNA Microarray Analysis
(A) Genes that were upregulated by the P683S mutation in the SC5314 genetic background (MRR1F5 versus MRR1F2–1). The genes are ordered according to their average degree of upregulation in four repeat experiments performed with two independent transformants (strains A and B). The changes in the expression level of these genes in the drug-resistant clinical isolate F5 as compared with a matched susceptible isolate (F5 versus F1) and in two independently constructed mrr1Δ mutants (strains A and B) as compared with their wild-type progenitor F5 (F5mrr1Δ versus F5) are shown for comparison.
(B) Genes that were upregulated by the G997V mutation in the SC5314 genetic background (MRR1G5 versus MRR1G2–2). The genes are ordered according to their average degree of upregulation in four repeat experiments performed with two independent transformants (strains A and B). The changes in the expression level of these genes in the drug-resistant clinical isolate G5 as compared with a matched susceptible isolate (G5 versus G1) and in two independently constructed mrr1Δ mutants (strains A and B) as compared with their wild-type progenitor G5 (mrr1Δ versus G5) are shown for comparison.
The fold increase or decrease in the expression of the genes is given for each experiment. Upregulated genes are highlighted in red, downregulated genes are highlighted in green, and genes that were not differentially expressed are shown as white boxes in all pairwise comparisons.