The Transcription Factor Mrr1p Controls Expression of the MDR1 Efflux Pump and Mediates Multidrug Resistance in Candida albicans
Figure 5
MRR1 Gain-of-Function Alleles Cause Constitutive MDR1 Overexpression and Multidrug Resistance
(A) MICs (in μg ml−1) of the indicated metabolic inhibitors for the wild-type parental strain SC5314, two independently constructed homozygous mrr1Δ mutants, and derivatives carrying the indicated mutant MRR1 alleles in the absence or presence of a wild-type MRR1 allele.
(B) MDR1 promoter activity in a wild-type reporter strain, an mrr1Δ mutant, and derivatives carrying the indicated mutant MRR1 alleles in the absence or presence of a wild-type MRR1 allele. The strains were grown to log phase in YPD medium and the mean fluorescence of the cells was determined by flow cytometry.