Two Plasmodium Rhomboid Proteases Preferentially Cleave Different Adhesins Implicated in All Invasive Stages of Malaria
Figure 5
Cleavage of Rh Proteins by Parasite Rhomboid Enzymes
GFP-tagged Rh1 (A), Rh2a (B), Rh2b (C), and Rh4 (D) were tested for cleavage by rhomboid enzymes by analyzing conditioned media with anti-GFP from transiently transfected COS cells. TgROM5 and PfROM4 readily cleaved each Rh protein, while PfROM1 could not cleave Rh2a but cleaved other Rh proteins at a lower efficiency. Note that in many cases smaller GFP-Rh protein breakdown products were readily detected in conditioned media (unpublished data). Molecular weight standards in kDa are denoted to the right of each panel.