Nuclear Retention of Multiply Spliced HIV-1 RNA in Resting CD4+ T Cells
Figure 5
Induction of Virus Production by PTB or Tat
High-level expression of PTB or Tat in resting CD4+ T cells from patients on HAART induces virus production that is comparable to that seen following PHA stimulation. Resting CD4+ T cells purified from patients on HAART were transfected as described above with expression vectors for PTB or Tat, or as a positive control, were stimulated with PHA. For all transfections, supernatant HIV-1 levels were measured at 72 h post-stimulation. For PHA-stimulated cells, supernatant levels of HIV-1 RNA were measured every day for a 6-d period. Levels shown reflect peak virus release over the 6-d period.