Listeria monocytogenes Invades the Epithelial Junctions at Sites of Cell Extrusion
Figure 7
L. monocytogenes Invasion of the Intestinal Epithelium at the Villus-Tip Extrusion Zone
(A) Optical longitudinal section through a villus tip from Wt-GFP L. monocytogenes infected rabbit intestinal tissue stained with phalloidin for F-actin (red) and with toto-3 for nuclei (blue). GFP-expressing bacteria are shown in green.
(B) Three-dimensional reconstruction of villus tips viewed from the lumenal side from tissue stained as in (A).
(C) Optical cross-section through three-dimensional reconstruction of a villus tip revealing intracellular L. monocytogenes (green). F-actin (red) is stained with phalloidin. Right: enlarged view of infected cell revealing actin nucleation on the surface of Wt-GFP L. monocytogenes.
(D) Three-dimensional confocal reconstruction of villus tip from tissue stained with antibodies to ZO-1 (red) and with toto-3 for nuclei (blue). Arrow indicates a cell being extruded.
(E) Three-dimensional reconstruction of infected villus tip stained with antibodies to ZO-1 (red), showing a multicellular junction.
Scale bars 10 μm.