Broad and durable protection against SARS-CoV-2 and SARS-CoV by an intranasal chimpanzee adenovirus vaccine expressing tandem RBDs and nucleocapsid

Yaping Liu; Runhong Zhou; Tian Zhao; Ruoke Wang; Kun Zhu; Junxian Hong; Ziqing Yang; Qianqian Yang; Yuqing Lei; Yang Bai; Jing Wei; Peng Chen; Xinyu Fan; Qi Zhang; Xuanling Shi; Peng Liu; Zhiwei Chen; Linqi Zhang

doi:10.1371/journal.ppat.1014436

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Open Access

Peer-reviewed

Research Article

Broad and durable protection against SARS-CoV-2 and SARS-CoV by an intranasal chimpanzee adenovirus vaccine expressing tandem RBDs and nucleocapsid

Yaping Liu ,

Contributed equally to this work with: Yaping Liu, Runhong Zhou

Roles Conceptualization, Formal analysis, Investigation, Project administration, Resources, Visualization, Writing – original draft

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Runhong Zhou ,

Contributed equally to this work with: Yaping Liu, Runhong Zhou

Roles Formal analysis, Investigation, Resources, Visualization, Writing – original draft, Funding acquisition

Affiliations AIDS Institute, Department of Microbiology and Pandemic Research Alliance Unit, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China, Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, Hong Kong Special Administrative Region, People’s Republic of China, Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People’s Republic of China
⨯
Tian Zhao,

Roles Investigation, Resources

Affiliation School of Biomedical Engineering, Tsinghua University, Beijing, China
⨯
Ruoke Wang,

Roles Investigation, Resources

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Kun Zhu,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Junxian Hong,

Roles Investigation

Affiliations Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China, Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
⨯
Ziqing Yang,

Roles Formal analysis, Visualization

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Qianqian Yang,

Roles Formal analysis, Visualization

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Yuqing Lei,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Yang Bai,

Roles Investigation

Affiliation School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Jing Wei,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Peng Chen,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Xinyu Fan,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Qi Zhang,

Roles Funding acquisition

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Xuanling Shi,

Roles Funding acquisition

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China
⨯
Peng Liu,

Roles Writing – review & editing

Affiliation School of Biomedical Engineering, Tsinghua University, Beijing, China
⨯
Zhiwei Chen ,

Roles Conceptualization, Funding acquisition, Writing – review & editing

* E-mail: zchenai@hku.hk (ZC); zhanglinqi@tsinghua.edu.cn (LZ)

Affiliations AIDS Institute, Department of Microbiology and Pandemic Research Alliance Unit, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China, Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, Hong Kong Special Administrative Region, People’s Republic of China, Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People’s Republic of China
⨯
[ ... ],
Linqi Zhang

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: zchenai@hku.hk (ZC); zhanglinqi@tsinghua.edu.cn (LZ)

Affiliations Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China, Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China, Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen, China

https://orcid.org/0000-0003-4931-509X

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Broad and durable protection against SARS-CoV-2 and SARS-CoV by an intranasal chimpanzee adenovirus vaccine expressing tandem RBDs and nucleocapsid

Yaping Liu,
Runhong Zhou,
Tian Zhao,
Ruoke Wang,
Kun Zhu,
Junxian Hong,
Ziqing Yang,
Qianqian Yang,
Yuqing Lei,
Yang Bai

Published: July 24, 2026
https://doi.org/10.1371/journal.ppat.1014436

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