O-GlcNAcylation licenses RNF166 to degrade the M protein of porcine coronaviruses

Runhui Qiu; Yucheng Zhang; Junwei Zhou; Jianan Wang; Wenbing Tang; Xinliang Xie; Yan Cheng; Tong Ding; Peng Sun; Yuting Shi; Caili Xi; Yanrong Zhou; Liurong Fang; Shaobo Xiao

doi:10.1371/journal.ppat.1014301

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Open Access

Peer-reviewed

Research Article

O-GlcNAcylation licenses RNF166 to degrade the M protein of porcine coronaviruses

Runhui Qiu,

Roles Conceptualization, Investigation, Validation, Writing – original draft, Writing – review & editing

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Yucheng Zhang,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Junwei Zhou,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Jianan Wang,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Wenbing Tang,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Xinliang Xie,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Yan Cheng,

Roles Validation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Tong Ding,

Roles Validation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Peng Sun,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Yuting Shi,

Roles Investigation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Caili Xi,

Roles Validation

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Yanrong Zhou,

Roles Project administration, Writing – original draft

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Liurong Fang ,

Roles Conceptualization, Project administration, Supervision, Writing – review & editing

* E-mail: vet@mail.hzau.edu.cn (SX), fanglr@mail.hzau.edu.cn (LF)

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China
⨯
Shaobo Xiao

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

* E-mail: vet@mail.hzau.edu.cn (SX), fanglr@mail.hzau.edu.cn (LF)

Affiliations National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China

https://orcid.org/0000-0003-0023-9188

⨯

O-GlcNAcylation licenses RNF166 to degrade the M protein of porcine coronaviruses

Runhui Qiu,
Yucheng Zhang,
Junwei Zhou,
Jianan Wang,
Wenbing Tang,
Xinliang Xie,
Yan Cheng,
Tong Ding,
Peng Sun,
Yuting Shi

Published: June 25, 2026
https://doi.org/10.1371/journal.ppat.1014301

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