Structural basis for sarbecovirus Rc-o319 spike adaptation to Rhinolophus cornutus Bat ACE2 and constraints on switching to human ACE2

Jingjing Wang; Zexuan Li; Yong Ma; Zimu Li; Hang Yuan; Chuanying Niu; Yifeng Teng; Banghui Liu; Mei Li; Min Zhou; Wenxiu Liu; Huimin Feng; Jing Chen; Jun He; Xinwen Chen; Xiaoli Xiong

doi:10.1371/journal.ppat.1014245

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Open Access

Peer-reviewed

Research Article

Structural basis for sarbecovirus Rc-o319 spike adaptation to Rhinolophus cornutus Bat ACE2 and constraints on switching to human ACE2

Jingjing Wang ,

Contributed equally to this work with: Jingjing Wang, Zexuan Li, Yong Ma

Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China
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Zexuan Li ,

Contributed equally to this work with: Jingjing Wang, Zexuan Li, Yong Ma

Roles Conceptualization, Investigation, Formal analysis, Methodology, Writing – original draft

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, University of Chinese Academy of Sciences, Beijing, China
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Yong Ma ,

Contributed equally to this work with: Jingjing Wang, Zexuan Li, Yong Ma

Roles Data curation, Formal analysis, Funding acquisition, Methodology, Visualization, Writing – original draft

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China
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Zimu Li,

Roles Methodology, Software

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China, Guangzhou National Laboratory, Guangzhou, China
⨯
Hang Yuan,

Roles Data curation, Resources, Writing – review & editing

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China, University of Chinese Academy of Sciences, Beijing, China
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Chuanying Niu,

Roles Data curation, Resources

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, University of Science and Technology of China, Hefei, China
⨯
Yifeng Teng,

Roles Investigation

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, University of Chinese Academy of Sciences, Beijing, China
⨯
Banghui Liu,

Roles Resources

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China
⨯
Mei Li,

Roles Data curation, Methodology

Affiliation Guangzhou National Laboratory, Guangzhou, China
⨯
Min Zhou,

Roles Data curation, Methodology

Affiliation Guangzhou National Laboratory, Guangzhou, China
⨯
Wenxiu Liu,

Roles Data curation, Methodology

Affiliation Guangzhou National Laboratory, Guangzhou, China
⨯
Huimin Feng,

Roles Data curation

Affiliation State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
⨯
Jing Chen,

Roles Writing – review & editing

Affiliation Guangzhou National Laboratory, Guangzhou, China
⨯
Jun He,

Roles Data curation, Methodology

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China
⨯
Xinwen Chen ,

Roles Conceptualization, Funding acquisition, Investigation, Supervision, Project administration, Writing – review & editing

* E-mail: chen_xinwen@gzlab.ac.cn (XC); xiong_xiaoli@gzlab.ac.cn (XX)

Affiliations Guangzhou National Laboratory, Guangzhou, China, State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
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[ ... ],
Xiaoli Xiong

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: chen_xinwen@gzlab.ac.cn (XC); xiong_xiaoli@gzlab.ac.cn (XX)

Affiliations State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Guangzhou Medical University-Guangzhou Institutes of Biomedicine and Health Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China, Guangzhou National Laboratory, Guangzhou, China

https://orcid.org/0000-0002-4632-9122

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Structural basis for sarbecovirus Rc-o319 spike adaptation to Rhinolophus cornutus Bat ACE2 and constraints on switching to human ACE2

Jingjing Wang,
Zexuan Li,
Yong Ma,
Zimu Li,
Hang Yuan,
Chuanying Niu,
Yifeng Teng,
Banghui Liu,
Mei Li,
Min Zhou

Published: May 21, 2026
https://doi.org/10.1371/journal.ppat.1014245

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