African swine fever virus pEP364R acts as an important inflammatory-inducing factor to activate NLRP3 inflammasome-mediated pyroptosis by regulating DDX3X

Qian Li; XuSheng Ma; XiaoFen Shang; XiaoFeng Nian; Ruoqing Mao; ZhiKuan Luo; ZiXiang Zhu; Yi Ru; Fan Yang; WeiJun Cao; YanYan Chang; YiGang Xu; Asad Jahangir; Syed Mubash-Sher Tajmir; XiaoHong Ma; YanYan Zhang; YuGuang Fu; JingJing Pei; HaiXue Zheng

doi:10.1371/journal.ppat.1013874

Loading metrics

Open Access

Peer-reviewed

Research Article

African swine fever virus pEP364R acts as an important inflammatory-inducing factor to activate NLRP3 inflammasome-mediated pyroptosis by regulating DDX3X

Qian Li ,

Contributed equally to this work with: Qian Li, XuSheng Ma

Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China
⨯
XuSheng Ma ,

Contributed equally to this work with: Qian Li, XuSheng Ma

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: maxusheng@caas.cn (XSM); 18843184112@163.com (YYZ); fuyuguang@caas.cn (YGF); peijingjing@caas.cn (JJP); zhenghaixue@caas.cn (HXZ)

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China

https://orcid.org/0009-0000-7047-5157

⨯
XiaoFen Shang,

Roles Formal analysis, Investigation, Visualization

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
XiaoFeng Nian,

Roles Funding acquisition, Investigation, Resources, Writing – original draft

Affiliation China-Malaysia National Joint Laboratory, Biomedical Research Center, Life Science and Engineering College, Northwest Minzu University, Lanzhou, China
⨯
Ruoqing Mao,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
ZhiKuan Luo,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
ZiXiang Zhu,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
Yi Ru,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
Fan Yang,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
WeiJun Cao,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
YanYan Chang,

Roles Project administration

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
YiGang Xu,

Roles Methodology

Affiliations College of Veterinary Medicine, Northeast Agricultural University, Harbin, China, Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science & Technology, Zhejiang A&F University, Hangzhou, P.R. China, Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, P.R. China
⨯
Asad Jahangir,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
Syed Mubash-Sher Tajmir,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
XiaoHong Ma,

Roles Methodology

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
YanYan Zhang ,

Roles Methodology, Resources, Supervision, Writing – review & editing

* E-mail: maxusheng@caas.cn (XSM); 18843184112@163.com (YYZ); fuyuguang@caas.cn (YGF); peijingjing@caas.cn (JJP); zhenghaixue@caas.cn (HXZ)

Affiliation Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China
⨯
YuGuang Fu ,

Roles Resources, Supervision, Writing – review & editing

* E-mail: maxusheng@caas.cn (XSM); 18843184112@163.com (YYZ); fuyuguang@caas.cn (YGF); peijingjing@caas.cn (JJP); zhenghaixue@caas.cn (HXZ)

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
JingJing Pei ,

Roles Resources, Supervision, Writing – review & editing

* E-mail: maxusheng@caas.cn (XSM); 18843184112@163.com (YYZ); fuyuguang@caas.cn (YGF); peijingjing@caas.cn (JJP); zhenghaixue@caas.cn (HXZ)

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
[ ... ],
HaiXue Zheng

Roles Funding acquisition, Project administration, Supervision, Writing – review & editing

* E-mail: maxusheng@caas.cn (XSM); 18843184112@163.com (YYZ); fuyuguang@caas.cn (YGF); peijingjing@caas.cn (JJP); zhenghaixue@caas.cn (HXZ)

Affiliations State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China
⨯
[ view all ]
[ view less ]

African swine fever virus pEP364R acts as an important inflammatory-inducing factor to activate NLRP3 inflammasome-mediated pyroptosis by regulating DDX3X

Qian Li,
XuSheng Ma,
XiaoFen Shang,
XiaoFeng Nian,
Ruoqing Mao,
ZhiKuan Luo,
ZiXiang Zhu,
Yi Ru,
Fan Yang,
WeiJun Cao

Published: February 25, 2026
https://doi.org/10.1371/journal.ppat.1013874

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Cytokines
Is the Subject Area "Cytokines" applicable to this article?

Thanks for your feedback.
Inflammation
Is the Subject Area "Inflammation" applicable to this article?

Thanks for your feedback.
Inflammasomes
Is the Subject Area "Inflammasomes" applicable to this article?

Thanks for your feedback.
Secretion
Is the Subject Area "Secretion" applicable to this article?

Thanks for your feedback.
Cell death
Is the Subject Area "Cell death" applicable to this article?

Thanks for your feedback.
Cell staining
Is the Subject Area "Cell staining" applicable to this article?

Thanks for your feedback.
Swine
Is the Subject Area "Swine" applicable to this article?

Thanks for your feedback.