A single DNA methylation site regulates cell fate during Clostridioides difficile sporulation

Pola Kuhn; John W. Ribis; Mi Ni; Gang Fang; Aimee Shen

doi:10.1371/journal.ppat.1013845

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Open Access

Peer-reviewed

Research Article

A single DNA methylation site regulates cell fate during Clostridioides difficile sporulation

Pola Kuhn,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America
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John W. Ribis,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Resources

Current address: Nikon Microscope Solutions, Boston, Massachusetts, United States of America.

Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America
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Mi Ni,

Roles Data curation, Formal analysis, Investigation

Affiliation Department of Genetics and Genomic Sciences, Institute for Genomics and Multiscale Biology, Mount Sinai School of Medicine, New York, New York, United States of America
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Gang Fang,

Roles Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing

Affiliation Department of Genetics and Genomic Sciences, Institute for Genomics and Multiscale Biology, Mount Sinai School of Medicine, New York, New York, United States of America
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Aimee Shen

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: aimee.shen@tufts.edu

Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America

https://orcid.org/0000-0002-9786-5742

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A single DNA methylation site regulates cell fate during Clostridioides difficile sporulation

Pola Kuhn,
John W. Ribis,
Mi Ni,
Gang Fang,
Aimee Shen

Published: July 23, 2026
https://doi.org/10.1371/journal.ppat.1013845

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Bacterial sporulation
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DNA methylation
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Cell cycle and cell division
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DNA transcription
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Cell membranes
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Gene expression
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Bacterial spores
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