Cryptosporidium oocyst wall proteins are true components of the oocyst wall and COWP8 is not required for parasite transmission

Ross Bacchetti; Sarah Stevens; Leandro Lemgruber; Mariana Azevedo Gonzalez Oliva; Emma M. Sands; Konstantinos Alexandrou; Michele Tinti; Lee Robinson; Jack C. Hanna; Simona Seizova; Peyton Goddard; Massimo Vassalli; Mattie Christine Pawlowic

doi:10.1371/journal.ppat.1013561

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Open Access

Peer-reviewed

Research Article

Cryptosporidium oocyst wall proteins are true components of the oocyst wall and COWP8 is not required for parasite transmission

Ross Bacchetti,

Roles Conceptualization, Investigation, Methodology, Visualization, Writing – original draft

Current address: Scottish Microbiology Reference Laboratories, Glasgow Royal Infirmary, Glasgow, United Kingdom

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Sarah Stevens,

Roles Conceptualization, Investigation, Visualization, Writing – original draft

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Leandro Lemgruber,

Roles Investigation, Visualization

Affiliation Cellular Analysis Facility, MVLS-Shared Research Facilities, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, United Kingdom
⨯
Mariana Azevedo Gonzalez Oliva,

Roles Investigation, Methodology

Current address: Institute for Bioengineering of Catalonia, Barcelona, Spain

Affiliation James Watt School of Engineering, University of Glasgow, Glasgow, United Kingdom
⨯
Emma M. Sands,

Roles Investigation

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Konstantinos Alexandrou,

Roles Visualization

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Michele Tinti,

Roles Formal analysis, Software, Visualization

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Lee Robinson,

Roles Investigation

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Jack C. Hanna,

Roles Methodology

Current address: Centre for Parasitology, School of Infection and Immunity, College of Medical, Veterinary, and Life Sciences, University of Glasgow, Glasgow, United Kingdom

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Simona Seizova,

Roles Methodology

Current address: Walter and Eliza Hall Institute of Medical Research, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Melbourne, Victoria, Australia

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Peyton Goddard,

Roles Validation

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom
⨯
Massimo Vassalli,

Roles Conceptualization, Resources, Supervision

Affiliation James Watt School of Engineering, University of Glasgow, Glasgow, United Kingdom
⨯
Mattie Christine Pawlowic

Roles Conceptualization, Funding acquisition, Investigation, Supervision, Visualization, Writing – original draft

* E-mail: mcpawlowic@dundee.ac.uk

Affiliation Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom

https://orcid.org/0000-0003-2002-6681

⨯

Cryptosporidium oocyst wall proteins are true components of the oocyst wall and COWP8 is not required for parasite transmission

Ross Bacchetti,
Sarah Stevens,
Leandro Lemgruber,
Mariana Azevedo Gonzalez Oliva,
Emma M. Sands,
Konstantinos Alexandrou,
Michele Tinti,
Lee Robinson,
Jack C. Hanna,
Simona Seizova

Published: October 23, 2025
https://doi.org/10.1371/journal.ppat.1013561

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